Active Recombinant Mouse NGFR protein, hFc-tagged

• Cat.No.: NGFR-940M
• Product Overview: Recombinant Mouse NGFR protein(Q9Z0W1)(Met1-Asn253), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 2 ng/mL Recombinant mouse NGF.
• Molecular Mass: The secreted recombinant mouse NGFR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a calculated molecular mass of 50.6 kDa. The apparent molecular mass of rmNGFR/Fc monomer is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Length: Met1-Asn253
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ngfr nerve growth factor receptor (TNFR superfamily, member 16) [ Mus musculus ]
• Official Symbol: NGFR
• Synonyms: NGFR; nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16; NGF receptor; p75 neurotrophin receptor; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor; p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16
• Gene ID: 18053
• mRNA Refseq: NM_033217
• Protein Refseq: NP_150086