Active Recombinant Mouse NOTCH1 Protein, His-tagged

• Cat.No.: NOTCH1-884M
• Product Overview: Recombinant mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed,with a C-terminal polyhistidine tag.

Specification

• Source: Baculovirus-Insect Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ala 19
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly1, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Immobilized mouse DLL4-his at 10 μg/mL (100 μl/
• Molecular Mass: The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >82 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Notch1 Notch gene homolog 1 (Drosophila) [ Mus musculus ]
• Official Symbol: NOTCH1
• Synonyms: NOTCH1; Notch gene homolog 1 (Drosophila); neurogenic locus notch homolog protein 1; mT14; p300; Motch A; notch 1; major type A protein; transmembrane receptor Notch1; N1; Mis6; Tan1; lin-12; 9930111A19Rik
• Gene ID: 18128
• mRNA Refseq: NM_008714
• Protein Refseq: NP_032740
• Pathway: Activated NOTCH1 Transmits Signal to the Nucleus, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Dorso-ventral axis formation, organism-specific biosystem; Dorso
• Function: chromatin DNA binding; chromatin binding; protein binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity