Recombinant mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed,with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly1, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Immobilized mouse DLL4-his at 10 μg/mL (100 μl/
The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>82 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.