Active Recombinant Mouse Notch1 protein, His-tagged

• Cat.No.: Notch1-422M
• Product Overview: Recombinant Mouse Notch1(Met 1-Gln 526) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Source: Insect cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ala 19
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human DLL4 at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL.3. Immobilized mouse DLL4-his at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL.
• Molecular Mass: The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
• Protein length: Met 1-Gln 526
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 82 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Notch1 Notch gene homolog 1 (Drosophila) [ Mus musculus ]
• Official Symbol: Notch1
• Synonyms: NOTCH1; Notch gene homolog 1 (Drosophila); neurogenic locus notch homolog protein 1; mT14; p300; Motch A; notch 1; major type A protein; transmembrane receptor Notch1; N1; Mis6; Tan1; lin-12; 9930111A19Rik
• Gene ID: 18128
• mRNA Refseq: NM_008714
• Protein Refseq: NP_032740
• UniProt ID: Q01705
• Chromosome Location: 2 A3; 2 18.91 cM
• Pathway: Activated NOTCH1 Transmits Signal to the Nucleus, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Dorso-ventral axis formation, organism-specific biosystem; Dorso-ventral axis formation, conserved biosystem; Gene expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem;
• Function: chromatin DNA binding; chromatin binding; protein binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity