Recombinant mouse Prdx1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Prdx1, also known as peroxiredoxin-1, is an important member of peroxiredoxins (Prdxs) regulating various cellular signaling and differentiation. It confers an aggressive survival phenotype of cancer cells and drug-resistance. This protein is also identified as a red blood cell factor which enhances natural killer (NK) cell activity.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Specific activity is >2,500 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for minute.
23.2kDa (207aa)18-28KDa (SDS–PAGE under reducing conditions.)
MSSGNAKIGY PAPNFKATAV MPDGQFKDIS LSEYKGKYVV FFFYPLDFTF VCPTEIIAFS DRADEFKKLN CQVIGASVDS HFCHLAWINTPKKQGGLGPM NIPLISDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITINDLPVG RSVDEIIRLV QAFQFTDKHG EVCPAGWKPGSDTIKPDVNK SKEYFSKQKL EHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)