Recombinant Mouse Proc(Ile19-Leu460 (pro) & Ala42-Leu460 (mature)) fused with His tag at C-terminal was expressed in CHO.
Predicted N Terminal:
Ile19 & Ala42
Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Measured by its ability to cleave the fluorogenic peptide substrate, Boc-beta -benzyl-DPR-AMC. The specific activity is >125 pmol/min/µg.
Predicted Molecular Mass: 49 (Pro) & 48 (Mature) kDa;
SDS-PAGE: 70 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.