Active Recombinant Mouse Sphk1 protein, Met/His-tagged

• Cat.No.: Sphk1-526M
• Product Overview: Recombinant Mouse Sphk1(Glu2-Pro382) fused with an N-terminal Met and 6-His tag was expressed in Insect cells.

Specification

• Source: Insect cells
• Species: Mouse
• Tag: His
• Form: Supplied as a 0.2 μm filtered solution in MES, NaCl, Glycerol and DTT.
• Bio-activity: Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine). The specific activity is >2,500 pmol/min/μg.
• Molecular Mass: Predicted Molecular Mass: 43 kDa;SDS-PAGE: 42 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: Sphk1 sphingosine kinase 1 [ Mus musculus ]
• Official Symbol: Sphk1
• Synonyms: SPHK1; sphingosine kinase 1; SK 1; SPK 1; Sk1; Spk1; 1110006G24Rik
• Gene ID: 20698
• mRNA Refseq: NM_001172472
• Protein Refseq: NP_001165943
• UniProt ID: Q8CI15
• Chromosome Location: 11; 11 E2
• Pathway: Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: ATP binding; D-erythro-sphingosine kinase activity; DNA binding; calmodulin binding; calmodulin binding; diacylglycerol kinase activity; kinase activity; magnesium ion binding; nucleotide binding; protein phosphatase 2A binding; sphinganine kinase activity; sphinganine kinase activity; transferase activity