Recombinant Mouse Sphk1(Glu2-Pro382) fused with an N-terminal Met and 6-His tag was expressed in Insect cells.
Supplied as a 0.2 μm filtered solution in MES, NaCl, Glycerol and DTT.
Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine). The specific activity is >2,500 pmol/min/μg.
Predicted Molecular Mass: 43 kDa;SDS-PAGE: 42 kDa, reducing conditions;
<1.0 EU per 1 μg of the protein by the LAL method.
>70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.