Active Recombinant Mouse Spock3 protein, His-tagged

• Cat.No.: Spock3-871M
• Product Overview: Recombinant Mouse Spock3 protein(NP_076178.1)(Met1-Ile436), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-Leu-Arg-AMC.The IC50 value is < 15 nM.
• Molecular Mass: The recombinant mouse Spock3 consists 425 amino acids and predicts a molecular mass of 48.3 kDa.
• Protein Length: Met1-Ile436
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Spock3 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 [ Mus musculus ]
• Official Symbol: Spock3
• Synonyms: SPOCK3; sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3; testican-3; testican 3; AI428471; mKIAA4039; 2900045C01Rik; KIAA4039
• Gene ID: 72902
• mRNA Refseq: NM_001252620
• Protein Refseq: NP_001239549