Recombinant mouse TNFSF10 (P50592) (Pro 118-Asn 291) was expressed, with a polyhistide tag at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.51, 5%~8% trehalose and mannitol.
The recombinant mouse TNFSF10 consisting of 190 amino acids and has a calculated molecular mass of 22.2 kDa. rm TNFSF10 migrates as an approximately 22 KDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.