Recombinant mouse Tnfrsf11B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Tnfrsf11b, also known as tumor necrosis factor receptor superfamily member 11B, is a member of the tumor necrosis factor receptor TNFR superfamily. It inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Also It plays a role in preventing arterial calcification.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
44.4kDa (388aa)40-57kDa (SDS-PAGE under reducing conditions)
ETLPPKYLHY DPETGHQLLC DKCAPGTYLK QHCTVRRKTL CVPCPDHSYT DSWHTSDECV YCSPVCKELQ SVKQECNRTH NRVCECEEGRYLEIEFCLKH RSCPPGSGVV QAGTPERNTV CKKCPDGFFS GETSSKAPCI KHTNCSTFGL LLIQKGNATH DNVCSGNREA TQKCGIDVTLCEEAFFRFAV PTKIIPNWLS VLVDSLPGTK VNAESVERIK RRHSSQEQTF QLLKLWKHQN RDQEMVKKII QDIDLCESSV QRHLGHSNLTTEQLLALMES LPGKKISPEE IERTRKTCKS SEQLLKLLSL WRIKNGDQDT LKGLMYALKH LKTSHFPKTV THSLRKTMRF LHSFTMYRLYQKLFLEMIGN QVQSVKISCL LEHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.25mg/ml (determined by Absorbance at 280nm)