Recombinant Mouse Vegfa Protein

Cat.No.: Vegfa-720M
Product Overview: Recombinant Mouse VEGF120 Ala 27 - Arg 146 (Accession # AAB22254.1) was produced in human 293 cells (HEK293).
Source: HEK293
Species: Mouse
Predicted N Terminal: Ala 27
Form: Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse VEGF120 at 2 μg/mL (100 μl/well) can bind Human VEGF R1, His Tag with a linear range of 3-50 ng/mL.
Molecular Mass: Mouse VEGF120 contains no "tag", and has a calculated MW of 17.2 kDa. The reducing protein migrates as 18-22 kDa in SDS-PAGE due to glycosylation.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Vegfa vascular endothelial growth factor A [ Mus musculus ]
Official Symbol: Vegfa
Synonyms: VEGFA; vascular endothelial growth factor A; vascular permeability factor; Vpf; Vegf; Vegf120; Vegf164; Vegf188;
Gene ID: 22339
mRNA Refseq: NM_001025250
Protein Refseq: NP_001020421
MIM:
UniProt ID:
Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem;
Function: cell surface binding; chemoattractant activity; cytokine activity; fibronectin binding; growth factor activity; heparin binding; platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity; receptor agonist activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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