Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant P. multocida toxA Protein, his-tagged

Recombinant P. multocida toxA Protein, his-tagged

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His(2)
Cat.No.:
toxA-001P
Product Overview:
This product is derived from a recombinant P. multocida ToxA with His6-tag at N-terminal expressed in E. coli.
Description:
Pasteurella multocida toxin (PMT) is produced by a gram-negative bacillus, Pasteurella multocida. PMT activates the Gq and G12/13 dependent signaling pathways. Gq and G12/13 are alpha subunits of the GTP trimer bound protein of animal cells. It does not activate the highly related G11-dependent pathways. This toxin binds to a ganglioside-type cell surface receptor, acts intracellularly after having been internalized through an endocytic pathway, and has pleiotropic effects on cell physiology. Therefore, it does not function on receptor-deficient cells or cells defective in the endocytosis pathway.
Source:
E. coli
Species:
P. multocid
Tag :
His
Form:
50% glycerol, 5mM Tris-HCl (pH7.5), 0.1M NaCl
Molecular Mass:
145 kDa
Purity:
More than 90% purity by SDS-PAGE (CBB staining)
Applications:
1) For the studies on the GTP trimer bound protein- dependent signaling pathway.
2) As a PMT antigen for immunological assays such as ELISA, western blotting and dot blotting.
Storage:
Ship at 4centigrade or -20centigrade and store at -20centigrade (or -80centigrade for long period).
Warning:
Wear safety glasses, protective clothing, and rubber or latex gloves. and when handling hypodermic needles avoid inadvertent selfinoculation. Do not pipette by mouth. Avoid inhalation of this product. This product is to be used by skilled personnel in a laboratory setting only. Good laboratory technique should be employed. This product is for research purposes only. It is not for use in humans and is not to be used as a diagnostic agent.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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