Recombinant Phleum pratense PHLPII was expressed in E. coli.
Phl p 2 is a lyophilized, recombinant protein with IgE-binding capacity. It was produced by heterologous expression in E. coli, purified by conventional biochemical methods.
yophilized from 5 mM PO4 buffer, pH 7.4
When stored at -20 centigrade the quality of the material will be maintained for several years. However, for short periods (max. 3 weeks) the lyophilized product may be kept at room temperature. After reconstitution store at -20 centigrade. Avoid repeated freezing/thawing.
The material can be reconstituted with distilled water (or equivalent) or dilute buffers. Thorough agitating during dissolution is essential. Do not use salt conzentrations exceeding 20 mM to dissolve the lyophilized material. Salt may be added after dissolution.If reconstituted to 1 mg/mL the product will be soluble to at least 99% and the PO4 concentration will be 1.4 mM.