Recombinant Phleum pratense Phlp5 was expressed in E. coli.
Phl p 5 is a lyophilized, recombinant protein with IgE-binding capacity. It was produced by heterologous expression in E. coli, purified by conventional biochemical methods.
lyophilized from sodium phosphate buffer (Ph 7.4)
When stored at -20 centigrade the quality of the material will be maintained for several years. However, for short periods (max. 3 weeks) the lyophilized product may be kept at room temperature. After reconstitution store at -20 centigrade. Avoid repeated freezing/thawing.
The material should be reconstituted with distilled water (or equivalent) or dilute buffers. Do not use salt concentrations exceeding 20 mM to dissolve the lyophilized material. Salt may be added after dissolution. Gentle agitation during dissolution is essential, afterwards incubate for 30 min to allow a complete reconstitution of the protein. If reconstituted with distilled water to a concentration of 1 mg/ml, the phosphate concentration will be 4 mM. At this concentration, the product is soluble to more than 99%.