Active Recombinant Pig IL6 protein

• Cat.No.: IL6-1025P
• Product Overview: Recombinant Pig IL6 protein(NP_999564.1)(Pro29-Met212) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Pig
• Tag: N/A
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized sus scrofa (pig) IL6 at 2 μg/ml (100 μl/well) can bind Human IL-6R-His, the EC50 of Human IL-6R-His is 250-750 ng/mL. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-5 ng/mL.
• Molecular Mass: The recombinant sus scrofa IL6 consists 185 amino acids and predicts a molecular mass of 21.2 kDa.
• Protein Length: Pro29-Met212
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Sus scrofa ]
• Official Symbol: IL6
• Synonyms: IL6; interleukin 6 (interferon, beta 2); interleukin-6; IL-6; interleukin-6 protein
• Gene ID: 399500
• mRNA Refseq: NM_214399
• Protein Refseq: NP_999564