Active Recombinant Pig TF Protein, His-tagged

• Cat.No.: TF-569P
• Product Overview: Recombinant pig (Sus scrofa) transferrin (NP_001231582.1) (Met 1-Thr 715) was fused with the a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Pig
• Tag: His
• Predicted N Terminal: Gln 22
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind human His-TFRC (cat: 11020-H07H) functional ELISA.2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is
• Molecular Mass: The secreted recombinant Sus scrofa (Pig) transferrin comprises 705 amino acids and has a predicted molecular mass of 78.3 kDa. It migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TF transferrin [ Sus scrofa ]
• Official Symbol: TF
• Synonyms: TF; transferrin; serotransferrin; siderophilin; beta-1 metal-binding globulin
• Gene ID: 396996
• mRNA Refseq: NM_001244653
• Protein Refseq: NP_001231582
• Pathway: Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem