Recombinant Pig TF Protein, His-tagged

Cat.No.: TF-569P
Product Overview: Recombinant pig (Sus scrofa) transferrin (NP_001231582.1) (Met 1-Thr 715) was fused with the a polyhistidine tag at the C-terminus.
Source: HEK293
Species: Pig
Tag: His
Predicted N Terminal: Gln 22
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its ability to bind human His-TFRC (cat: 11020-H07H) functional ELISA.2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is
Molecular Mass: The secreted recombinant Sus scrofa (Pig) transferrin comprises 705 amino acids and has a predicted molecular mass of 78.3 kDa. It migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: TF transferrin [ Sus scrofa ]
Official Symbol: TF
Synonyms: TF; transferrin; serotransferrin; siderophilin; beta-1 metal-binding globulin;
Gene ID: 396996
mRNA Refseq: NM_001244653
Protein Refseq: NP_001231582
MIM:
UniProt ID:
Pathway: Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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