Recombinant Porcine FCGRT(Asp 16 - Ser 289)/B2M(Val 21 - His 118), subunit FCGRT / FcRN is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Asp 16 (FcRN) & Val 21 (B2M)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
Has a calculated MW of 32.3 kDa (FCGRT / FcRN) and 12.8 kDa (B2M). The reducing (R) protein migrates as 36 kDa (FCGRT) and 15 kDa (B2M) respectively.
FCGRT(Asp 16 - Ser 289)/B2M(Val 21 - His 118)
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
See Certificate of Analysis for reconstitution instructions and specific concentrations.