Recombinant rat Btla (NP_998795.1) (Ala6-Tyr183) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. 2. Immobilized rat BTLA-mFc at 10 μg/mL (100 μL/well) can bind mouse HVEM-Fch. The EC50 of mouse EFNA5-Fc is 6.2-14.4 ng/mL.
The recombinant rat Btla consists 384 amino acids and predicts a molecular mass of 43.4 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.