Recombinant Rat CD4 Protein, His-tagged
Recombinant rat Cd4 (NP_036837.1) (Met1-Thr394) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10^4 cells/well areadded to ratCD4-His coated plates (0.2μg/mL and 100μL/well), approximately >70% will adhere specifically aft
The recombinant rat Cd4 consists 378 amino acids and predicts a molecular mass of 42.5 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.