Recombinant rat EFNB1 (P52796) extracellular domain (Met 1-Thr 229) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.51, 5%~8% trehalose and mannitol.
Immobilized rat EFNB1-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse EphB3-His, The EC50 of biotinylated mouse EphB3-His is 6.3-14.6 ng/ml.
The recombinant rat EFNB1 comprises 216 amino acids and predicts a molecular mass of 23.8 kDa. The apparent molecular mass of the ratEFNB1 is approximately 36 kDa in SDS-PAGE under reducing conditions due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.