Active Recombinant Rat EFNB1 Protein, His-tagged

• Cat.No.: EFNB1-1077R
• Product Overview: Recombinant rat EFNB1 (P52796) extracellular domain (Met 1-Thr 229) was expressed, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Predicted N Terminal: Ala 25
• Form: Lyophilized from sterile PBS, pH 7.51, 5%~8% trehalose and mannitol.
• Bio-activity: Immobilized rat EFNB1-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse EphB3-His, The EC50 of biotinylated mouse EphB3-His is 6.3-14.6 ng/ml.
• Molecular Mass: The recombinant rat EFNB1 comprises 216 amino acids and predicts a molecular mass of 23.8 kDa. The apparent molecular mass of the ratEFNB1 is approximately 36 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Efnb1 ephrin B1 [ Rattus norvegicus ]
• Official Symbol: EFNB1
• Synonyms: EFNB1; ephrin B1; ephrin-B1; ELK-L; LERK-2; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; LERK2
• Gene ID: 25186
• mRNA Refseq: NM_017089
• Protein Refseq: NP_058785
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: ephrin receptor binding; ephrin receptor binding; ephrin receptor binding; protein binding