Active Recombinant Rat EFNB2 Protein, Fc-tagged

• Cat.No.: EFNB2-1078R
• Product Overview: Recombinant rat EFNB2 (NP_001100798.1) extracellular domain (Met 1-Ala 229) was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Predicted N Terminal: Ser 28
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EPHB4-His at 10 μg/mL (100 μl/well) can bind Rat EphrinB2-Fc, The EC50 of Rat EphrinB2-Fc is 10 ng/mL.
• Molecular Mass: The recombinant rat EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and predicts a molecular mass of 49 kDa. The apparent molecular mass of the rat EFNB2/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Efnb2 ephrin B2 [ Rattus norvegicus ]
• Official Symbol: EFNB2
• Synonyms: EFNB2; ephrin B2
• Gene ID: 306636
• mRNA Refseq: NM_001107328
• Protein Refseq: NP_001100798
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: ephrin receptor binding; ephrin receptor binding; receptor binding