Recombinant rat EFNB2 (NP_001100798.1) extracellular domain (Met 1-Ala 229) was fused with the Fc region of human IgG1 at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EPHB4-His at 10 μg/mL (100 μl/well) can bind Rat EphrinB2-Fc, The EC50 of Rat EphrinB2-Fc is 10 ng/mL.
The recombinant rat EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and predicts a molecular mass of 49 kDa. The apparent molecular mass of the rat EFNB2/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.