"Ephb3" Related Products

Recombinant Rat Ephb3 Protein, His-tagged

Cat.No.: Ephb3-1160R
Product Overview: Recombinant rat Ephb3 (NP_001099338.1) (Met1-Thr537) was expressed with a polyhistidine tag at the C-terminus.
Source: HEK293
Species: Rat
Tag: His
Predicted N Terminal: Leu 30
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rat EPHB3-His at 10 μg/ml (100 μl /well) can bind rat EFNB1-Fc, The EC50 of rat EFNB1-Fc is 2-6ng/ml.
Molecular Mass: The recombinant rat Ephb3 consists 519 amino acids and predicts a molecular mass of 57 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Ephb3 Eph receptor B3 [ Rattus norvegicus ]
Official Symbol: Ephb3
Synonyms: EPHB3; Eph receptor B3; ephrin type-B receptor 3; EPH-like tyrosine kinase-2;
Gene ID: 287989
mRNA Refseq: NM_001105868
Protein Refseq: NP_001099338
MIM:
UniProt ID:
Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem;
Function: ATP binding; axon guidance receptor activity; axon guidance receptor activity; ephrin receptor activity; ephrin receptor activity; nucleotide binding; protein tyrosine kinase activity; receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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