Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Rat Fcgrt/B2m protein, His/Strep II/Avi-tagged, Biotinylated

Recombinant Rat Fcgrt/B2m protein, His/Strep II/Avi-tagged, Biotinylated

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Cat.No.:
Fcgrt & B2m-1537R
Product Overview:
Recombinant Rat FCGRT(Ala 23 - Ser 298)/B2M(Ile 21 - Met 119), the subunit FCGRT / FcRN carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Strep II tag at the C-terminus was expressed in HEK293.
Source:
HEK293
Species:
Rat
Tag:
His/Strep II/Avi
Predicted N Terminal:
Ala 23 (FcRN) & Ile 21 (B2M)
Form:
Lyophilized from 0.22um filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Molecular Mass:
The protein has a calculated MW of 34.7 kDa (FcRN), 13.1 kDa (B2M) . The protein migrates as 45-50kDa (FCGRT), 14 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Protein length:
FCGRT(Ala 23 - Ser 298)/B2M(Ile 21 - Met 119)
Endotoxin:
Less than 1.0 EU per μg by the LAL method.
Purity:
>95% as determined by SDS-PAGE.
Notes:
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Storage:
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 200 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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