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Active Recombinant Rat Fcgrt & B2m protein, Avi-His&StrepII-tagged, Biotinylated

Cat.No. : Fcgrt & B2m-1537R
Product Overview : Biotinylated Recombinant Rat Fcgrt & B2m protein(Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M)), fused with Avi-His and StrepII tag, was expressed in HEK293.
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Source : HEK293
Species : Rat
Tag : Avi-His&StrepII
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag captured on Biotin CAP-Series S Sensor Chip can bind Herceptin with an affinity constant of 10.3 nM as determined in a SPR assay (Biacore T200).
Molecular Mass : Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.
Protein Length : Ala23-Ser298(FCGRT)&Ile21-Met119(B2M)
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Fcgrt Fc fragment of IgG, receptor, transporter, alpha [ Rattus norvegicus ]
Official Symbol : FCGRT
Synonyms : FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; neonatal Fc receptor; Fc fragment immunoglobulin G receptor; Fc receptor, IgG, alpha chain transporter; igG Fc fragment receptor transporter alpha chain; FcRn;
Gene ID : 29558
mRNA Refseq : NM_033351
Protein Refseq : NP_203502

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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10/16/2019

    With a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.

    07/24/2019

      It maintains its activity and effectiveness under various environmental conditions.

      04/11/2017

        The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.

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