Fcgrt & B2m-1537R
Recombinant Rat Fcgrt/B2m protein, His/Strep II/Avi-tagged, Biotinylated
Recombinant Rat FCGRT(Ala 23 - Ser 298)/B2M(Ile 21 - Met 119), the subunit FCGRT / FcRN carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Strep II tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Ala 23 (FcRN) & Ile 21 (B2M)
Lyophilized from 0.22um filtered solution in PBS (pH 7.4) with Trehalose as protectant.
The protein has a calculated MW of 34.7 kDa (FcRN), 13.1 kDa (B2M) . The protein migrates as 45-50kDa (FCGRT), 14 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
FCGRT(Ala 23 - Ser 298)/B2M(Ile 21 - Met 119)
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitute at 200 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.