Active Recombinant Rat Hmgb1 protein, LPS-Free

• Cat.No.: Hmgb1-19R
• Product Overview: Recombinant Rat Hmgb1 was expressed in E. coli.

Specification

• Source: E. coli
• Species: Rat
• Form: 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT.
• Bio-activity: Measured by its ability to induce migration. Maximal activity in the cell migration assay is obtained at 1 nM, or 25 ng/ml.
• Molecular Mass: Has a calculated molecular mass of approximately 24.8 kDa. It migrates at a position of approximately 30 kD in SDS-PAGE gels, possibly because of the unusual number of positively charged amino acids it contains.
• Protein length: HMGB1 consists of 215 amino acid residues.
• AA Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE
• Endotoxin: <0.4 ng LPS per mg protein
• Purity: The purified protein is >95% homogeneous (electrophoresis and mass spectrometry). It contains no nucleic acids, nor IL-1beta.
• Storage: Once resuspended can be stored frozen at -20 centigrade. To avoid cysteine oxidation, DTT 0.5mM is added during protein purification.

Gene Information

• Gene Name: Hmgb1 high mobility group box 1 [ Rattus norvegicus ]
• Official Symbol: Hmgb1
• Synonyms: HMGB1; high mobility group box 1; high mobility group protein B1; HMG-1; amphoterin; high mobility group 1; heparin-binding protein p30; high mobility group protein 1; Hmg1; Ac2-008; MGC93598; MGC93599
• Gene ID: 25459
• mRNA Refseq: NM_012963
• Protein Refseq: NP_037095
• UniProt ID: P63159
• Chromosome Location: 12p11
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem
• Function: 5S rRNA binding; DNA binding, bending; DNA binding, bending; RAGE receptor binding; RAGE receptor binding; bent DNA binding; calcium-dependent protein kinase regulator activity; crossed form four-way junction DNA binding; cytokine activity; cytokine activity; double-stranded DNA binding; four-way junction DNA binding; glycolipid binding; heparin binding; heparin binding; open form four-way junction DNA binding; peptide binding; protein binding; protein dimerization activity; protein kinase activator activity; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; single-stranded DNA binding; transcription factor binding; transcription factor binding