"IL12A/IL12B" Related Products

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Recombinant Rat IL12A & IL12B Protein, His-tagged

Cat.No.: IL12A & IL12B-1257R
Product Overview: Recombinant rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the p
Source: HEK293
Species: Rat
Tag: His
Predicted N Terminal: Val 22 & Met 23
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 2-15 ng/mL.
Molecular Mass: The recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: (17.9+53.5+2.9+23.8) % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Il12a interleukin 12A [ Rattus norvegicus ]
Official Symbol: IL12A
Synonyms: IL12A; interleukin 12A; interleukin-12 subunit alpha; IL-12A; CLMF p35; IL-12 subunit p35; interleukin 12 p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit;
Gene ID: 84405
mRNA Refseq: NM_053390
Protein Refseq: NP_445842
MIM:
UniProt ID:
Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved
Function: cytokine activity; contributes_to cytokine activity; growth factor activity; contributes_to growth factor activity; interleukin-12 beta subunit binding; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; interleu

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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