Recombinant rat Il17f (NP_001015011.1) (Met1-Ala153) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. 2. Immobilized rat IL17F-His at 10 μg/mL (100 μL/well) can bind Rat IL17RA-Fc3. The EC50 of Rat IL17RA-Fc3 is 0.28-0.66 μg/mL.
The recombinant rat Il17f consists 145 amino acids and predicts a molecular mass of 16.4 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.