Recombinant rat Il9 (NP_001099217.1) (Met1-Ala144) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, 5%~8% trehalose and mannitol.
Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 19.8-79.2 ng/mL.
The recombinant rat Il9 consists 137 amino acids and predicts a molecular mass of 15.6 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.