Recombinant Rat Lcn2(21-198aa) fused with His tag at N-terminal was expressed in E. coli.
Lcn2 also known as Neutrophil gelatinase-associated lipocalin. It belongs to the calycin superfamily and Lipocalin family. It is essential component of the antimicrobial innate immune system, is present in neutrophils and multiple other tissues. It prevents iron acquisition by microorganisms by sequestering iron-loaded bacterial siderophores. In contrast to its pro-apoptotic activity, Lcn2 has also been reported to be a survival factor. Lcn2 protected thyroid carcinoma cells from apoptosis induced by serum deprivation and silencing of Lcn2 reduced thyroid tumor growth.
Liquid. In Phosphate Buffered Saline (pH7.4) containing 20% glycerol
22.9kDa (201aa) confirmed by MALDI-TOF
MGSSHHHHHH SSGLVPRGSH MGSQDSTQNL IPAPPLISVP LQPGFWTERF QGRWFVVGLA ANAVQKERQS RFTMYSTIYE LQEDNSYNVTSILVRGQGCR YWIRTFVPSS RPGQFTLGNI HSYPQIQSYD VQVADTDYDQ FAMVFFQKTS ENKQYFKVTL YGRTKGLSDE LKERFVSFAKSLGLKDNNIV FSVPTDQCID N
> 95% by SDS - PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.25mg/ml (determined by Absorbance at 280nm)