Active Recombinant Rat Pdpn protein(Met1-Leu135), hFc-tagged

• Cat.No.: Pdpn-1149R
• Product Overview: Recombinant Rat Pdpn (NP_062231.1) (Met1-Leu135) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-hFc
• Protein length: Met1-Leu135
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized RatPDPN-Fc at 10 μg/mL (100 μL/well) can bind CLEC1B-His/Biotin, the EC50 of human CLEC1B-His/Biotin is 300-600 ng/mL.
• Molecular Mass: The recombinant Rat Pdpn consists 351 amino acids and predicts a molecular mass of 38.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pdpn podoplanin [ Rattus norvegicus ]
• Official Symbol: Pdpn
• Synonyms: PDPN; podoplanin; T1A; RTI140; glycoprotein 38; E11 antigen epitope; type I cell 40 kDa protein; epithelial cell surface transmembrane protein antigen; pulmonary type I alveolar epithelial cell transmembrane differentiation marker; E11; Gp38; OTS-8; RTI40; T1-alpha
• Gene ID: 54320
• mRNA Refseq: NM_019358
• Protein Refseq: NP_062231