Recombinant rat TIMP1 (P01860) (Met1-Ala217) was expressed and purified.
Predicted N Terminal:
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.0, 5%~8% trehalose and mannitol.
Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGLDPA-AR-NH2. The IC50 value is approximately 4 nM.
The recombinant rat TIMP1 consists of 194 amino acids and has a predicted molecular mass of 21.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>97 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.