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Active Recombinant Rat TNF protein

Cat.No. : TNF-1071R
Product Overview : Recombinant Rat TNF protein(P16599)(Leu80-Leu235) was expressed in E. coli.
  • Specification
  • Gene Information
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Source : E. coli
Species : Rat
Tag : N/A
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-6 pg/ml.
Molecular Mass : The recombinant rat TNFα consists of 157 amino acids and has a calculated molecular mass of 17.4 kDa. It migrates as an approximately 15.9 kDa band in SDS-PAGE under reducing conditions.
Protein Length : Leu80-Leu235
Purity : > 93 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Tnf tumor necrosis factor [ Rattus norvegicus ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; Tnfa; RATTNF; TNF-alpha; MGC124630;
Gene ID : 24835
mRNA Refseq : NM_012675
Protein Refseq : NP_036807

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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