Recombinant Rhesus EPHA4 Protein, Fc-tagged
Recombinant rhesus EPHA4 (NP_001247799.1) (Met1-Thr547) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA.Immobilized Rhesus EPHA4 at 10 μg/mL (100 μl/well) can bind biotinylated Rhesus EFNA5-Fc. The EC50 of biotinylated Rhesus EFNA5-Fc is 14.4-33.6 ng/mL.
The recombinant rhesus EPHA4 comprises 769 amino acids and has a calculated molecular mass of 85.4KDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>96 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.