Recombinant rhesus EPHA4 (NP_001247799.1) (Met1-Thr547) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA. Immobilized Rhesus EPHA4-His at 10 μg/ml (100 μl/well) can bind mouse EFNA5-Fc, The EC50 of mouse EFNA5-Fc is 28.2-65.8 ng/ml.
The recombinant rhesus EPHA4 comprises 539 amino acids and has a calculated molecular mass of 59.8 KDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.