Recombinant rhesus IGFBP1 (XP_001085935.2) (Met23-Asn281) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA. Immobilized Rhesus IGFBP1-His at 10 μg/ml (100 μl/well) can bind biotinylated human IGF1 with a linear range of 12.5-100 ng/ml.
The recombinant rhesus comprises 245 amino acids and has a calculated molecular mass of 26.7 KDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>98 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.