Active Recombinant Rhesus PDCD1 protein(Met1-Gln167), hFc-tagged

• Cat.No.: PDCD1-1221R
• Product Overview: Recombinant Rhesus PDCD1 (NP_001107830.1) (Met1-Gln167) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rhesus
• Tag: C-hFc
• Protein length: Met1-Gln167
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human PD-L1 His at 2 μg/ml (100 μl/well) can bind Rhesus PD-1 hFc, the EC50 of Rhesus PD-1 hFc is 40-160 ng/mL.
• Molecular Mass: The recombinant rhesus PD1 consists 381 amino acids and predicts a molecular mass of 42.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.