Recombinant rhesus TNFSF10 (NP_001252963.1) (Val114-Gly281) was expressed and purified with an initial Met.
Predicted N Terminal:
Lyophilized from sterile PBS1, 5%~8% trehalose and mannitol.
1. Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-2 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Rhesu
The recombinant rhesus TNFSF10 consists of 169 amino acids and has a calculated molecular mass of 19.6 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>98 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.