Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant SARSV Papain-like Protease protein

Recombinant SARSV Papain-like Protease protein

   Share
"Papain-like Protease" Related Products.
By Species
SARSV(1)
By Source
E. coli(1)
Cat.No.:
Papain-like Protease-207
Product Overview:
Recombinant SARSV Papain-like Protease(Glu1541 - Lys1841) was expressed in E. coli.
Description:
The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons. PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro. When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species. This protein contains an N-terminal 6-His tag.
Source:
E. coli
Species:
SARSV
Form:
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
Bio-activity:
Recombinant SARS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate. Using tetra-Ubiquitin chains as a substrate, PLPro demonstrates a preference for K48 linkages when using a 100 nM enzyme concentration--at higher enzyme concentration other linkages are cleaved as well.
Molecular Mass:
Predicted Molecular Mass: 37 kDa
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.