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Home > Products > Recombinant Proteins > Recombinant SARSV Papain-like Protease protein

Recombinant SARSV Papain-like Protease protein

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E. coli(1)
Papain-like Protease-207
Product Overview:
Recombinant SARSV Papain-like Protease(Glu1541 - Lys1841) was expressed in E. coli.
The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons. PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro. When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species. This protein contains an N-terminal 6-His tag.
E. coli
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
Recombinant SARS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate. Using tetra-Ubiquitin chains as a substrate, PLPro demonstrates a preference for K48 linkages when using a 100 nM enzyme concentration--at higher enzyme concentration other linkages are cleaved as well.
Molecular Mass:
Predicted Molecular Mass: 37 kDa
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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