SNAP-25 is a recombinant protein substrate for C. botulinum Types A and E Neurotoxin. It fused with affinity tag and expressed in E. coli.
The protein was recombinantly expressed in E. coli and purified using affinity and ion exchange chromatography. The GST affinity tag has been cleaved off of the protein prior to quantitation and pachaging.
Each vial, when reconsitituted with 100 ul of water contains 100 ul of SNAP-25 in 20 Mm HEPES, pH 7.4, 1.25% lactose.
This product is not known to be toxic
This product is supplied as a lyophilized powder which has been stoppered under vacuum. Store lyophilized vials at 2-8 centigrade. Once dissolved, aliquot and store the product at -20 centigrade. Refrain from multiple freeze/thaw cycles.
Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.