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Recombinant Toxoplasma gondii GRA1 protein

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E. coli(2)
Cat.No.:
GRA1-889T
Product Overview:
E.coli derived recombinant. The protein contains the P24 (GRA1) immunodominant regions (57-149aa).
Description:
The life cycle of Toxoplasma gondii has two phases. The coccidia like takes place only in members of the Felidae family which makes these animals the parasite's primary host. The asexual part of the life cycle can take place in any warm-blooded animal, like other mammals(including felines) and birds. T. gondii constructing daughter scaffolds within the mother cell. In the intermediate hosts (including felines), the parasite invades cells, forming intracellular so-called parasitophorous vacuoles containing bradyzoites, the slowly replicating form of the parasit. Vacuoles form tissue cysts mainly within the muscles and brain. Since they are within cells, the host's immune system does not detect these cysts. Resistance to antibiotics varies, but the cysts are very difficult to eradicate entirely. Within these vacuoles T. gondii propagates by a series of binary fissions until the infected cell eventually bursts and tachyzoites are released. Tachyzoites are the motile, asexually reproducing form of the parasite. Unlike the bradyzoites, the free tachyzoites are usually efficiently cleared by the host's immune response, although some manage to infect cells and form bradyzoites, thus maintaining the infection.
Source:
E. coli
Species:
Toxoplasma gondii
Protein length:
aa57-149
Purity:
>90% pure; Purity of proteins is evaluated by SDS-PAGE
Applications:
ELISA, WB, Flow-Through
Storage:
LongTerm: -80°C; Short Term: 4°C
Concentration:
~ 1 mg/ml; bulk amount available upon request.
Storage buffer:
1.5 M Urea; 20 mM Tris-HCLl pH 7.2; 50% glycerol.
Shipping:
Wet Ice


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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