Recombinant Xenopus laevis ranbp1 protein, His-tagged
Recombinant Xenopus laevis ranbp1 protein fused with His tag was expressed in bacterial.
This protein is an effector of Ran and binds with high affinity to Ran-GTP and a much lower affinity to Ran-GDP.
The biological activity of His-RanBP1 is verified by its ability to bind to constitutively active Ran but not to dominant negative Ran.
Quantitation of Ran-GTP in vitro and in vivo;Inhibition of Ran-GTP in vitro and in vivo.