Recombinant bacterial Cas9 protein, His-tagged

• Cat.No.: cas9-85H
• Product Overview: Recombinant bacterial Cas9 (1367 aa) fused with His Tag at N-terminal and nuclei translocation signal (NTS) was inserted at C-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Streptococcus pyogenes serotype M1
• Tag: His
• Form: 0.4 g/ml, sterile-filtered, in 50 mM Tri-HCl (pH 8.0), with 400 mM NaCl and 20% Glycerol
• Molecular Mass: 163.7 KD
• AA Sequence: MKHHHHHHQEFRSDKKYSIGLDIGT NSVGWAVITDEYKVPSKKFKVLGNT DRHSIKKNLIGALLFDSGETAEATR LKRTARRRYTRRKNRICYLQEIFSN EMAKVDDSFFHRLEESFLVEEDKKH ERHPIFGNIVDEVAYHEKYPTIYHL RKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQL VQTYNQLFEENPINASGVDAKAILS ARLSKSRRLENLIAQLPGEKKNGLF GNLIALSLGLTPNFKSNFDLAEDAK LQLSKDTYDDDLDNLLAQIGDQYAD LFLAAKNLSDAILLSDILRVNTEIT KAPLSASMIKRYDEHHQDLTLLKAL VRQQLPEKYKEIFFDQSKNGYAGYI DGGASQEEFYKFIKPILEKMDGTEE LLVKLNREDLLRKQRTFDNGSIPHQ IHLGELHAILRRQEDFYPFLKDNRE KIEKILTFRIPYYVGPLARGNSRFA WMTRKSEETITPWNFEEVVDKGASA QSFIERMTNFDKNLPNEKVLPKHSL LYEYFTVYNELTKVKYVTEGMRKPA FLSGEQKKAIVDLLFKTNRKVTVKQ LKEDYFKKIECFDSVEISGVEDRFN ASLGTYHDLLKIIKDKDFLDNEENE DILEDIVLTLTLFEDREMIEERLKT YAHLFDDKVMKQLKRRRYTGWGRLS RKLINGIRDKQSGKTILDFLKSDGF ANRNFMQLIHDDSLTFKEDIQKAQV SGQGDSLHEHIANLAGSPAIKKGIL QTVKVVDELVKVMGRHKPENIVIEM ARENQTTQKGQKNSRERMKRIEEGI KELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDV DHIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLN AKLITQRKFDNLTKAERGGLSELDK AGFIKRQLVETRQITKHVAQILDSR MNTKYDENDKLIREVKVITLKSKLV SDFRKDFQFYKVREINNYHHAHDAY LNAVVGTALIKKYPKLESEFVYGDY KVYDVRKMIAKSEQEIGKATAKYFF YSNIMNFFKTEITLANGEIRKRPLI ETNGETGEIVWDKGRDFATVRKVLS MPQVNIVKKTEVQTGGFSKESILPK RNSDKLIARKKDWDPKKYGGFDSPT VAYSVLVVAKVEKGKSKKLKSVKEL LGITIMERSSFEKNPIDFLEAKGYK EVKKDLIIKLPKYSLFELENGRKRM LASAGELQKGNELALPSKYVNFLYL ASHYEKLKGSPEDNEQKQLFVEQHK HYLDEIIEQISEFSKRVILADANLD KVLSAYNKHRDKPIREQAENIIHLF TLTNLGAPAAFKYFDTTIDRKRYTS TKEVLDATLIHQSITGLYETRIDLS QLGGDGLYETRIDLSQLGGDSRADP KKKRKV
• Endotoxin: < 1 EU/ug
• Purity: >96% by SDS-PAGE
• Applications: 1. May be used for in vitro Cas9 mediated mammalian cell gene editing study by intracellular delivery this protein with target gene specific gRNA either together as complex or separagely with "ProFectin" reagent.2. Highly purified Cas9 antigen, may be used for specific antibody production.
• Storage: Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 15 days.