Flexible: Quantify hydrogen peroxide in solution, in cell extracts or directly from certain cellsVersatile: Detect a variety of oxidase activities through enzyme-coupled reactionsSensitive: Detect as little as 10 picomoles of hydrogen peroxideComplete: Mix and read reagents, with minimal hands-on timeConvenient: Homogenous assay format, fully compatible with HTS automation
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized "mix and read" assay that is suitable for both 96-and 348-well formats and is compatible with HTS liquid handling.
Fluorescence microscopy, HTS
Red Peroxidase Substrate (1 vial)Horseradish Peroxidase (HRP) (20 units)Hydrogen Peroxide (H2O2) Stock Solution (200 µl)DMSO (1 ml)Assay Buffer (100 ml)