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Home > Products > Assay Kits > Detection Kits > S-Nitrosylated Protein Detection Kit

S-Nitrosylated Protein Detection Kit

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Cat.No.:
Kit-2027
Size:
1 ea
Description:
Nitric oxide (NO) is produced by three distinct isoforms of nitric oxide synthase and functions as a key signaling molecule in physiology and pathophysiology. NO transduces its effects by reacting either directly with heme and non-heme centers of proteins or indirectly via further oxidation to various reactive nitrogen species (RNS). The S-Nitrosylated Protein Detection Assay employs a modification of the Jaffrey et al. Biotin-switch method to allow for the direct visualization of S-NO proteins in whole cells or tissues, as well as by western blot analysis. Using this method, free SH groups are first blocked (an addition of 125.1 amu per residue) and any S-NO bonds present in the sample are then cleaved. Biotinylation of the newly formed SH groups (an addition of 523.6 amu per residue) provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection.
Storage:
-20°C
Kit Components:
1S-Nitrosylation Wash Buffer (10X): 1 vial/25 ml; Room Temperature S-Nitrosylation Buffer: A 1 vial/30 ml; Room Temperature S-Nitrosylation Buffer B (5X): 1 vial/60 ml; Room Temperature S-Nitrosylation Blocking Reagent: 3 vials; -20°CS-Nitrosylation Labeling Reagent: 3 vials; -20°CS-Nitrosylation Reducing Reagent: 3 vials; Room TemperatureS-Nitrosylation Detection Reagent I (HRP): 1 vial; -20°CS-Nitrosylation Detection Reagent II (Fluorescein): 1 vial; -20°C


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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