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Home > Products > Assay Kits > Activity Kits > SET7/9 Methyltransferase Inhibitor Screening Assay Kit

SET7/9 Methyltransferase Inhibitor Screening Assay Kit

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Cat.No.:
Kit-2021
Description:
SET7/9 is an MT that acts on various substrates including histone 3 at lysine residue 4 (H3K4), p53, and the transcription factor TAF 10. In the SET7/9 MT Inhibitor Screening Assay the transfer of the methyl group from SAM by SET7/9 to the acceptor peptide (TAF 10) generates SAH, which is rapidly converted to urate and H2O2 using an enzyme mixture provided in the kit. A subsequent reaction between H2O2 and ADHP produces the highly fluorescent compound resorufin.
Storage:
-80°C
Kit Components:
MT Assay Buffer: 1 vial/20 ml; -20°CMT Assay Buffer Additive: 1 vial/0.2 ml; -20°C MT Enzyme Mixture: 3 vials/0.3 ml; -80°CMT Fluorometric Mixture: 3 vials; -20°CMT Assay S-Adenosylmethionine: 3 vials; -80°CSET7/9 (human recombinant): 2 vials/0.1 ml; -80°CDMSO Assay Reagent: 1 vial/1 ml; Room temperature HCl Assay Reagent (20 mM): 1 vial/1 ml; -20°CSET7/9 Acceptor Peptide: 2 vials/0.6 ml; -20°CMT Assay AdoHcy Positive Control: 1 vial/200 µl; -80°CHalf Volume 96-Well Solid Plate (black): 1 plate; Room temperature 96-Well Cover Sheet: 1 cover; Room temperature


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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