Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Soluble Epoxide Hydrolase Inhibitor Screening Assay Kit

Soluble Epoxide Hydrolase Inhibitor Screening Assay Kit

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Cat.No.:
Kit-2029
Size:
96 wells
Description:
Soluble epoxide hydrolase (sEH) catalyzes the hydrolysis of exogenous and endogenous epoxides to vicinal diols. Endogenous substrates for sEH include epoxyeicosatrienoic acids (EETs) which exhibit vasodialatory and anti-inflammatory activity. Small molecule inhibitors of sEH may therefore hold promise as therapeutics for the treatment of hypertension and vascular inflammation. The fluorescence-based sEH Inhibitor Screening Assay Kit provides a convenient method for screening epoxide hydrolase inhibitors. The assay utilizes (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester (PHOME) as a substrate. Hydrolysis of PHOME by epoxide hydrolase produces the highly fluorescent 6-methoxy-2-naphthaldehyde which can be analyzed using an excitation wavelength of 330 nm and emission wavelength of 465 nm. Each kit contains buffer, substrate, and sufficient human recombinant sEH for 100 tests.
Storage:
-80°C
Kit Components:
sEH Assay Buffer (10X): 1 vial/3 ml; -20°C sEH (human recombinant): 2 vials/12 µl; -80°CsEH Substrate: 1 vial/600 µl; -80°C96-Well Solid Plate (black): 1 plate; Room temperature 96-Well Cover Sheet: 1 cover; Room temperature


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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