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Home > Products > Assay Kits > Activity Kits > Sorbitol Dehydrogenase Colorimetric Assay Kit

Sorbitol Dehydrogenase Colorimetric Assay Kit

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Cat.No.:
Kit-0778
Product Overview:
Sorbitol Dehydrogenase Assay Kit (Colorimetric) is used for quantitative determination of Sorbitol Dehydrogenase activity using colourmetric methods.
Description:
SORBITOL DEHYDROGENASE (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.This non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Applications:
SDH activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)
Usage:
For research use only (RUO)
Storage:
Store all components at -20 °C upon receiving.
Kit Components:
Assay Buffer 10 mLDiaphorase 120 µLSubstrate 250 µLCalibrator 1.5 mLNAD/MTT Solution 1 mL
Detection method:
Colorimetric
Compatible Sample Types:
Culture media, Plasma, Serum, Tissue, Urine
Features & Benefits:
Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 125 U/L for 12 min reaction.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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