Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Metabolism Kits > Sphingomyelin Colorimetric Assay Kit

Sphingomyelin Colorimetric Assay Kit

"Sphingomyelin" Related Products.
Product Overview:
Sphingomyelin Assay Kit (Colorimetric) is used for measuring sphingomyelin using colorimetric methods.
Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. Sphingomyelin Assay Kit (Colorimetric) provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate.The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM.
Sphingomyelin quantification in various biological samplesAnalysis of cell signaling pathway
For research use only (RUO)
Store kit at -20°C, protected from light. Warm SM Assay Buffer to room temperature before use. Read the entire protocol before performing the assay
Kit Components:
SM Assay Buffer 100 mlOxiRed Probe 0.2 mlSphingomyelinase (Lyophilized) 1 vialALP Enzyme (Lyophilized) 1 vialSM Standard (5 mM) 50 μl
Detection method:
Compatible Sample Types:
Biological samples, Cell Samples, Tissue Samples

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.