Starch Assay Kit is used for measuring starch levels.
Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear α-1,4 linked polymer) and amylopectin, (highly α-1,6 branched α-1,4 polymer).Starch generally contains 0-25% amylose and 75-100% amylopectin. The Starch Assay Kit provides an easy, convenient method to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (λmax= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/mL.
For research use only (RUO)
Store kit at -20°C, protect from light and moisture. Warm Buffers to room temperature before use. Briefly centrifuge all small vials prior to opening. Read entire protocol before the assay.
Hydrolysis Buffer 25 mLDevelopment Buffer 25 mLOxiRed Probe 0.4 mLHydrolysis Enzyme Mix (lyophilized) 1 vialDevelopment Enzyme Mix (lyophilized) 1 vialStarch Standard (2.0 mg/mL) 100 μL
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine