Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Steatosis Colorimetric Assay Kit

Steatosis Colorimetric Assay Kit

   Share
"Steatosis" Related Products.
Cat.No.:
Kit-0806
Product Overview:
Steatosis Colorimetric Assay Kit provides a method for evaluating steatosis risk of drug candidates using Oil Red O to stain neutral lipids in hepatocytes.
Description:
Steatosis, also known as fatty liver, is a pathological process characterized by abnormal accumulation of lipid within cells. There are two distinct patterns of steatosis: macrovesicular and microvesicular. The former is frequently seen in alcohol-induced liver injury, as a complication of metabolic syndrome such as obesity and type II diabetes, and is a marker of the hepatotoxic side effect of certain drugs. Microvesicular steatosis is more commonly related to mitochondrial dysfunction and defects in β-oxidation responsible for fatty liver seen in pregnancy and Reye"s syndrome. While simple steatosis may not be associated with significant impairment of liver function, extensive fat accumulation can lead to cirrhosis and even liver failure. Studies on alcohol-induced steatosis revealed a set of transcription factors which are thought to be involved in the process, including SREBP1, PPARα, and Erg-1. The mechanism of non-alcoholic steatosis formation is poorly understood and little information is available on the pathway(s) responsible for progressive hepatocellular damage following lipid accumulation.
Usage:
For research use only (RUO)
Storage:
Fixative (10X) Room TemperatureWash Solution Room TemperatureOil Red O Solution 4°CDye Extraction Solution Room TemperatureChloroquine Positive Control (25 mM) 4°CSteatosis Assay Hematoxylin Room TemperatureThis kit will perform as specified if stored as directed and used before the expiration date indicated on theoutside of the box.
Kit Components:
Fixative (10X) 1 vial/10 mlWash Solution 6 vials/30 mlOil Red O Solution 1 vial/25 mlDye Extraction Solution 1 vial/30 mlChloroquine Positive Control (25 mM) 1 vial/100 µlSteatosis Assay Hematoxylin 1 vial/30 ml


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.