Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Succinate Dehydrogenase Activity Colorimetric Assay Kit

Succinate Dehydrogenase Activity Colorimetric Assay Kit

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Cat.No.:
Kit-0779
Product Overview:
Succinate Dehydrogenase Activity Assay Kit (Colorimetric) is used to measure Succinate Dehydrogenase activity.
Description:
Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone.SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species); therefore, measurement of succinate dehydrogenase activity has wide applications.Succinate Dehydrogenase Activity Assay kit is rapid, simple and high-throughput adaptable. In this assay, Succinate dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor (Probe), which changes the color from blue to a colorless product (depending upon the sample enzymatic activity).
Applications:
Measurement of Succinyl-CoA Dehydrogenase Activity in various tissues/cellsAnalysis of citric acid cycle
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C, protected from light.
Kit Components:
SDH Assay Buffer 25 mLSDH Substrate Mix (Lyophilized) 1 vialSDH Probe 0.2 mLDCIP Standard (2 mM) 0.4 mLSDH Positive Control (Lyophilized) 1 vial
Detection method:
Colorimetric
Compatible Sample Types:
Animal tissues: heart, liver, muscle, etc.Cell culture: adherent or suspension cellsPurified mitochondria


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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