"Sulfatase" Related Products

Sulfatase Colorimetric Activity Assay Kit

Cat.No.: Kit-2146
Product Overview: Sulfatase Activity Assay Kit provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at 515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.
Size: 100 assays
Description: Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer.
Applications: Measurement of sulfatase activity
Storage: Store kit at –20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.Sulfatase Assay Buffer, Sulfatase Substrate, Stop/Developing Solution, and 4-Nitrocatechol Standard: Bring to room temperature before use.Sulfatase: Reconstitute with 20 µl dH2O. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Stable for two months. Keep on iceduring use.
Kit Components: Sulfatase Assay Buffer: 5 mlSulfatase Substrate: 4 mlStop/Developing Solution: 10 mlSulfatase: 1 Vial4-Nitrocatechol Standard (0.5 mM): 1.5 ml
Detection method: Absorbance (515 nm)
Compatible Sample Types: • Animal tissues such as liver, endometrium, and breast cancer• Adherent or suspension cells• Purified enzyme
Features & Benefits: • Simple, Sensitive & Rapid protocol• Highly-Sensitive: Detects as low as 1 mU sulfatase in various sample types

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.